pCMV+LacI

BBa_I763008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I763008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alice Pasini
Date created: 2007-07-29 11:00:00
Date modified: 2015-08-31 04:08:08

LacI repressor from E. coli (+LVA) regulated by pCMV Promoter



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Generator

    Sequences BBa_I763008_sequence (Version 1)

    Description

    The costitutive promoter CMV regulates the expressione of LacI protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.

    Notes

    Intermediate

    Source

    Igem 2007 plates

    Access Instance Definition
    public
    public
    		BBa_J52034
    BBa_C0012
    		BBa_J52034
    lacI

    Sequence Annotation Location Component / Role(s)
    BBa_J52034
    BBa_C0012
    		1,654
    661,1788
    		BBa_J52034
    lacI

    igem#experience
    Fails
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I763008/1
     

    Attachments

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