Plamda-Lac

BBa_I763010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I763010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alice Pasini

Date created: 2007-08-19 11:00:00

Date modified: 2015-08-31 04:08:08

LacI repressor regulated by Plamda Promoter

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_I763010_sequence (Version 1)

Description

Promoter lambda - cI regulated - (R0051)regulates the expression of LacI reppression protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.
This biobrick is necessary to repress pLac activity in absence of enough endogenous LacI.

Notes

composite part

Source

Igem 2007 plates

Components

Access	Instance	Definition
public	BBa_R0051	cI lam
public	BBa_P0412	BBa_P0412

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0051	1,49	cI lam
BBa_P0412	58,1365	BBa_P0412

Other Properties

• igem#experience: Fails
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I763010/1

Member of these Collections

• iGEM Parts Registry