BBa_I766645

BBa_I766645 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I766645

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: alexander ng

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopJ-EE under weak constitutive promotor

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_I766645_sequence (Version 1)

Description

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pSte5 promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing a matching RR zipper. The effector is fused mCherry (RFP) on the N terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonucleases AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

composite part

Components

Access	Instance	Definition
public	BBa_I766557	BBa_I766557
public	BBa_I766101	BBa_I766101
public	BBa_I766002	BBa_I766002
public	BBa_I766102	BBa_I766102
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766557	1,601	BBa_I766557
BBa_I766101	608,1327	BBa_I766101
BBa_I766002	1336,2208	BBa_I766002
BBa_I766102	2217,2378	BBa_I766102
BBa_I766600	2387,2604	BBa_I766600

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766645/1

Member of these Collections

• iGEM Parts Registry