BBa_I766685

BBa_I766685 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I766685

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: alexander ng

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopJ-EE under inducible promotor

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_I766685_sequence (Version 1)

Description

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pFig promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing a matching RR zipper. The effector is fused mCherry (RFP) on the N terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonucleases AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

composite part

Components

Access	Instance	Definition
public	BBa_I766558	BBa_I766558
public	BBa_I766101	BBa_I766101
public	BBa_I766002	BBa_I766002
public	BBa_I766102	BBa_I766102
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766558	1,1000	BBa_I766558
BBa_I766101	1007,1726	BBa_I766101
BBa_I766002	1735,2607	BBa_I766002
BBa_I766102	2616,2777	BBa_I766102
BBa_I766600	2786,3003	BBa_I766600

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766685/1

Member of these Collections

• iGEM Parts Registry