BBa_I766998

BBa_I766998 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I766998
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jimmy Jie Min Huang
Date created: 2007-08-15 11:00:00
Date modified: 2015-08-31 04:08:10

YopJ-RR under inducible promotor



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_I766998_sequence (Version 1)

Description

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pFig1 promotor. The effector protein is fused to an RR leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

Composite Part.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I766998/1