Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oscar Hernandez and Matt Gemberling
Date created: 2005-09-14 11:00:00
Date modified: 2015-08-31 04:08:14
R0010 with Anti-Sense Theophillline Induced Antiswitch ("On Switch")
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_J04605_sequence (Version 1) |
Description
This part contains the R0010 Promoter region attached to an anti-sense antiswitch sequence for the regulation of EYFP This Promoter and Antiswitch construct is designed so that it can be attached to any form of the EYFP coding region. The purpose of this piece is to regulate the expression of YFP in the presence of theophylline. In this construct, the presence of theophylline causes the antiswitch, which is initially bound to the YFP coding region (preventing expression), to release and fold up on itself allowing for EYFP expression.Notes
Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html| Sequence Annotation | Location | Component / Role(s) |
| R0010 Promoter Region Hammerhead Riobozyme #1 Antiswitch Hammerhead Riobozyme #2 Antisense Binding Sequence | 1,200 201,253 254,308 309,359 254,268 | feature/promoter promoter stem_loop feature/stem_loop feature/misc sequence_feature feature/stem_loop stem_loop feature/binding non_covalent_binding_site |