Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas Cain, Davidson College
Date created: 2005-06-27 11:00:00
Date modified: 2015-08-31 04:08:14
Antiswitch repressing Biobrick coding sequence at the RBS ("Off" switch)
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_J04606_sequence (Version 1) |
Description
The presence of theophylline prevents translation of mRNA coding region directly downstream from any Biobrick that contains the BBa_B0034 RBS. This effect is the result of the antiswitch attaching to the B0034 RBS preceding the coding region. The absence of theophylline allows expression of the coding region as usual.Notes
Transcription of the antiswitch is repressed in the presence of tetR. Also, the actual RBS sequence of BBa_B0034 is only 12 bp long; three additional base pairs comprise the total target sequence, and these additional 3 bp's correspond to the last three base pairs of the B0034 Biobrick Prefix, Xba I.Source
Adaptation of antiswitch design "S1" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html| Sequence Annotation | Location | Component / Role(s) |
| R0010 Promoter Region Hammerhead Riobozyme #1 Antiswitch Antisense Binding Sequence Hammerhead Riobozyme #2 B0015 Terminator | 1,54 55,107 108,181 108,122 182,232 233,360 | promoter feature/promoter feature/stem_loop stem_loop feature/misc sequence_feature feature/binding non_covalent_binding_site feature/stem_loop stem_loop feature/misc sequence_feature |