Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas Cain, Davidson College
Date created: 2005-06-27 11:00:00
Date modified: 2015-08-31 04:08:15
Antiswitch enabling production of EYFP ("On" switch)
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_J04685_sequence (Version 1) |
Description
The presence of theophylline allows translation of mRNA transcribed from Biobrick BBa_E0032. No theophylline present causes repression of the coding region of BBa_E0032.Notes
Transcription of the antiswitch is repressed in the presence of tetR.Source
Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html| Sequence Annotation | Location | Component / Role(s) |
| R0010 Promoter Region Hammerhead Riobozyme #1 Antiswitch Antisense Binding Sequence Hammerhead Riobozyme #2 B0015 Terminator | 1,54 55,107 108,162 108,122 163,213 214,341 | feature/promoter promoter feature/stem_loop stem_loop feature/misc sequence_feature non_covalent_binding_site feature/binding feature/stem_loop stem_loop feature/misc sequence_feature |