Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas Cain, Davidson College
Date created: 2005-06-27 11:00:00
Date modified: 2015-08-31 04:08:15
Antiswitch repressing production of EYFP ("Off" switch)
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_J04686_sequence (Version 1) |
Description
The presence of theophylline prevents translation of mRNA transcribed from Biobrick BBa_E0032. The absence of theophylline allows expression of the coding region of BBa_E0032.Notes
Transcription of the antiswitch is repressed in the presence of tetRSource
Adaptation of antiswitch design "S1" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html| Sequence Annotation | Location | Component / Role(s) |
| R0010 Promoter Region Hammerhead Riobozyme #1 "On" Antiswitch Hammerhead Riobozyme #2 B0015 Terminator Antisense Binding Sequence | 1,54 55,107 108,181 182,232 233,360 108,122 | feature/promoter promoter stem_loop feature/stem_loop sequence_feature feature/misc feature/stem_loop stem_loop feature/misc sequence_feature feature/misc sequence_feature |