Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kristen DeCelle
Date created: 2005-09-14 11:00:00
Date modified: 2015-08-31 04:08:15
Part containing promoter, 8 bp, RBS, and riboswitch mTCT8-4 theophylline aptamer (J04705)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J04900_sequence (Version 1) |
Description
The specific parts contained within this part are (R0010. 8 bp. B0034. J04705). The riboswitch J04705 is designed to make or break a "kink" in the mRNA when theophylline is present. The ribosome should be obstructed in cases where there is a "kink" in the stem loop. When this happens translation should be turned off. We have ligated this part with yellow fluorescent protein (YFP) and a terminator and will test soon to determine whether the aptamer has a regulatory function on the production of YFP. The J04705 aptamer was taken from {R. Jenison, S. Gill, A. Pardi, B. Polisky, Science 263, 1426 (1994).} The 8 bp sequence was gotten from Gallivan and Desai supplemental material I believe. Gallivan and Desai used this same aptamer J04705, but added many extra bases. We are testing whether those bases are really necessary or whether one can just use the aptamer to regulate translation. The results from testing J04900 will hopefully tell us whether one can put the aptamer between the RBS and the coding region or whether translation is inhibited as we have heard.| Sequence Annotation | Location | Component / Role(s) |
| R0010 8 bp from Gallivan paper? RBS (B0034) Aptamer (J04705) | 1,200 201,208 209,220 221,258 | feature/promoter promoter sequence_feature feature/misc ribosome_entry_site feature/rbs sequence_feature feature/misc |