Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ytwang
Date created: 2005-06-13 11:00:00
Date modified: 2015-08-31 04:08:18
Lambda cI857 (E07700) (RBS- LVA+)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_J06502_sequence (Version 1) |
Description
Mutated from BBa_C0051 Repressor, Lambda cI (RBS- LVA+) to be temperature sensitive and UV insensitive.Notes
First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old.
Second attempt to mutation failed. The best we got was 2 out of 3 mutations. The sequencing had a lot of bad sequence at the beginings, so that the ind- mutation was not sequenced by either of the primers used. The ind- mutation was tested by cutting with HindIII.
This part is being put on hold. We may finish it by doing a single site mutation on one of the mutants with the other 2 sites successfully mutated.
Source
GenBank E00770 (Japanese Patent)| Sequence Annotation | Location | Component / Role(s) |
| cI857 LVA G->C G->A (ind-) C->G barcode from lambda cI (BBa_C0051) | 1,711 712,744 248,248 352,352 681,681 751,775 | CDS feature/cds tag feature/tag sequence_alteration feature/mutation feature/mutation sequence_alteration sequence_alteration feature/mutation engineered_tag feature/barcode |