BBa_J100030

BBa_J100030 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Scott Hall

Date created: 2011-08-31 11:00:00

Date modified: 2015-08-31 04:08:22

phoA is an inducible promoter induced by phosphate starvation.

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_J100030_sequence (Version 1)

Description

phoA should attach to the missing gap in the plasmid to allow transcription. This promoter is induced by phosphate starvation which means that it can limit protein synthesis. The source of phoA is E. Coli and this promoter is suitable for bacterial cells. phoA typically has 76 base pairs. phoA is inactive in its default state with high levels of phosphate. In the absence of phosphate, phoA is activated.

Source:
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Notes

Add CGCC to the 5' end of the bottom strand and add CGAC to the 5' end of the top strand.

Source

CATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTgTTTTTATTTTTTAATGTATTTGTAC
Source:
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Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100030/1

Member of these Collections

• iGEM Parts Registry