Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rebecca Evans
Date created: 2012-06-10 11:00:00
Date modified: 2015-08-31 04:08:22
Synthetic Riboswitch
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J100065_sequence (Version 1) |
Description
This part is a synthetic riboswitch that can be used in E. coli. In the presence of theophylline, the expression of the gene of interest is induced, but in the absence of theophylline, very little transcription takes place. This part contains a modified T5 promoter, which, in the absence of cymR, acts as a strong constitutive promoter. The ribosomal binding site is contained in the riboswitch.Notes
Because the riboswitch must be directly beside the gene of interest (the start codon is used in the folding of the riboswitch), we added a BsaI recognition site. This allows the riboswitch to be connected directly to a gene of interest using the Golden Gate Assembly method (if the first nucleotide after the start codon of the gene of interest is a C, we made it for use with superfolder GFP).Source
http://www.ncbi.nlm.nih.gov/pubmed?term=Synthetic%20Riboswitches%20That%20Induce%20Gene%20Expression%20in%20Diverse%20Bacterial%20Species| Sequence Annotation | Location | Component / Role(s) |
| BBa prefix T5 promoter lac operator scar constant sequence KpnI site Riboswitch RBS Start GGA prefix ATGC sticky word BBa suffix | 1,22 23,75 76,96 97,102 104,118 119,124 125,184 168,172 182,184 182,192 193,213 | engineered_region feature/BioBrick feature/promoter promoter feature/operator operator feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature ribosome_entry_site feature/rbs start_codon feature/start feature/misc sequence_feature engineered_region feature/BioBrick |