Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rebecca Evans
Date created: 2012-06-11 11:00:00
Date modified: 2015-08-31 04:08:22
Synthetic Riboswitch
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J100066_sequence (Version 1) |
Description
This part is a synthetic riboswitch that can be used in E. coli. In the presence of theophylline, the expression of the gene of interest is induced, but in the absence of theophylline, very little transcription takes place. This part contains a modified T5 promoter, which, in the absence of cymR, acts as a strong constitutive promoter. The ribosomal binding site is contained in the riboswitch.Because the riboswitch must be right beside the gene of interest (the start codon is folded in the riboswitch), we added a BsaI site. This allows the riboswitch to be attached to the gene of interest using Golden Gate Assembly (as long as the first nucleotide after the start codon of the gene of interest is a C, we designed the part to use with superfolder GFP).
This riboswitch is modified from riboswitch E as described in the paper by Topp et al.
References: Shana Topp, Colleen M. K. Reynoso, Jessica C. Seeliger, Ian S. Goldlust, Shawn K. Desai, Doroth??e Murat, Aimee Shen, Aaron W. Puri, Arash Komeili, Carolyn R. Bertozzi, June R. Scott, and Justin P. Gallivan. Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species. Applied and Environmental Microbiology, 76:23, 7881-7884. December 2010.
Notes
NoneSource
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988590/?tool=pubmed| Sequence Annotation | Location | Component / Role(s) |
| BBa prefix T5 promoter lac operator scar constant sequence KpnI site Riboswitch Start RBS GGA suffix ATGC sticky word BBa suffix | 1,22 23,75 76,96 97,102 104,118 119,124 125,187 185,187 168,174 185,195 196,216 | feature/BioBrick engineered_region feature/promoter promoter feature/operator operator sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/start start_codon feature/rbs ribosome_entry_site feature/misc sequence_feature feature/BioBrick engineered_region |