Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ben Clarkson
Date created: 2012-07-04 11:00:00
Date modified: 2015-08-31 04:08:22
cadA promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J100071_sequence (Version 1) |
Description
The promoter for the cadBA operon in E. coli. It is induced by low pH (cadA gene). CadC senses external pH, by low pH preventing the formation of a disulfide bond, activating CadC. When activated, CadC binds to two sites upstream of the cadBA promoter, Cad1 and Cad2. The binding of CadC to these sites activates transcription by releasing the H-NS molecules that are bound to, and repressing, the cadBA operon (CadC function in cadBA operon).Notes
We cloned the promoter region 334 bp upstream of the transcription start site, in which Cad1 and Cad2 are included.Source
Taken from E. coli genome through PCR.| Sequence Annotation | Location | Component / Role(s) |
| H-NS binding site H-NS binding site H-NS binding site H-NS binding site H-NS binding site H-NS binding site Cad1 binding site Cad2 binding site -10 -35 | 11,25 68,82 149,163 153,167 199,213 296,310 188,225 249,279 320,326 298,306 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site |