BBa_J100075

BBa_J100075 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100075

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Betsy Gammon

Date created: 2012-07-04 11:00:00

Date modified: 2015-08-31 04:08:22

CydAP1 Long Promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_J100075_sequence (Version 1)

Description

This promoter promotes the cydA and cydB genes. It is regulated by Fnr as well as arcA. While Fnr represses this promoter, phosphorylated arcA relieves this repression to induce the expression of the cydAB gene. This long version of the sequence includes 1 arcA binding site and 2 complete Fnr binding sites. In aerobic conditions, arcA is not phosphorylated, so Fnr represses the promoter. However, when arcA is phosphorylated in anaerobic conditions, the promoter is relived of the repression from Fnr.

Notes

The second Fnr site extends beyond the start of the transcription factor.

Source

E. coli

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
arcA binding site	35,84	feature/binding, non_covalent_binding_site
Fnr binding site 2	91,105	non_covalent_binding_site, feature/binding
Fnr binding site 1	143,158	non_covalent_binding_site, feature/binding
-35	116,116	feature/misc, sequence_feature
-10	141,141	sequence_feature, feature/misc
+1	151,151	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100075/1

Member of these Collections

• iGEM Parts Registry