Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Betsy Gammon
Date created: 2012-07-04 11:00:00
Date modified: 2015-08-31 04:08:22
CydAP1 Short Promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J100076_sequence (Version 1) |
Description
This promoter promotes the cydA and cydB genes. It is regulated by Fnr as well as arcA. While Fnr represses this promoter, phosphorylated arcA relieves this repression to induce the expression of the cydAB gene. This short version of the sequence includes 1 arcA binding site and 1 complete Fnr binding sites. In aerobic conditions, arcA is not phosphorylated, so Fnr represses the promoter. However, when arcA is phosphorylated in anaerobic conditions, the promoter is relived of the repression from Fnr.Notes
There is a second Fnr binding site; however it extends beyond the +1 site. Thus, this second binding site is incomplete, and Fnr will likely not be able to bind to it.Source
E. coli| Sequence Annotation | Location | Component / Role(s) |
| +1 -10 arcA binding site Fnr binding site -35 | 151,151 141,141 35,84 91,105 116,116 | feature/misc sequence_feature sequence_feature feature/misc non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/misc sequence_feature |