Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Betsy Gammon
Date created: 2012-07-11 11:00:00
Date modified: 2015-08-31 04:08:22
CydAP Long + RBS + GFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_J100084_sequence (Version 1) |
Description
This promoter promotes the cydA and cydB genes. It is regulated by Fnr as well as arcA. While Fnr represses this promoter, phosphorylated arcA relieves this repression to induce the expression of the cydAB gene. This long version of the sequence includes 1 arcA binding site and 2 complete Fnr binding sites. In aerobic conditions, arcA is not phosphorylated, so Fnr represses the promoter. However, when arcA is phosphorylated in anaerobic conditions, the promoter is relieved of the repression from Fnr. This promoter is attached to a medium strength RBS and a GFP gene.Notes
This part explicitly includes the full Fnr binding site.Source
E. coli| Access | Instance | Definition |
| public public | BBa_J100075 BBa_E0240 | BBa_J100075 GFP report |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J100075 BBa_E0240 | 1,158 167,1042 | BBa_J100075 GFP report |