BBa_J100085

BBa_J100085 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100085
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caroline Vrana
Date created: 2012-07-12 11:00:00
Date modified: 2015-08-31 04:08:22

short CRISPR sequence with GFP target spacer



    • Details

    Types
    DnaRegion

    Roles
    mature_transcript_region

    RNA

    Sequences BBa_J100085_sequence (Version 1)

    Description

    The CRISPR system (Clustered Regularly Interspaced Short Palindromic Repeats) acts as a bacterial immune system. This sequence is a synthetic CRISPR system used in E.coli. It includes a short 30 bp sequence that targets the GFP gene (E0040). It is cloned into the plasmid pSB1K8.

    Notes

    The CRISPR repeats made it difficult to not have inappropriate base pair matching with oligos.

    Source

    Oligos from a designed sequence.

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J100085/1
     

    Attachments

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