BBa_J100099

BBa_J100099 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100099

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Phoebe Parrish

Date created: 2012-09-12 11:00:00

Date modified: 2015-08-31 04:08:22

A promoter (CydAB) activated by the FNR enzyme

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_J100099_sequence (Version 1)

Description

The promoter, CydAB, was found to be activated by the FNR enzyme, which is induced by the presence of (NH4)2Fe(SO4)2 and ascorbate. The oligo includes both CydAB, the FNR binding site, and the sticky ends needed for the Golden Gate Assembly method.

Notes

We had to include the FNR binding site in our oligo.

Source

Info on FNR: "Activation of FNR-dependent transcription by iron: An in vitro switch for FNR" by Jeffrey Green and John R. Guest. FEMS Microbiology Letters vol. 113 (1993) pp. 219-222

Info on CydAB: "Transcriptional Control Mediated by the ArcA Two-Component Response Regulator Protein of Escherichia coli: Characterization of DNA Binding at Target Promoters" by A. Simon Lynch and Edmund C.C. Lin. Journal of Bacteriology, Nov. 1996, p. 6238???6249

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
FNR binding	1,22	feature/binding, non_covalent_binding_site
-35	29,34	feature/binding, non_covalent_binding_site
-10	53,58	feature/binding, non_covalent_binding_site

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100099/1

Member of these Collections

• iGEM Parts Registry