Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jessica Gronniger
Date created: 2013-06-18 11:00:00
Date modified: 2015-08-31 04:08:22
P5 promoter cloned into scaffold
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J100110_sequence (Version 1) |
Description
P5 promoter (J119031) ligated between junctions A and B on scaffold (J119301). Golden Gate assembly was used to put the promoter into the gap between junctions A and B. iPCR was used to generate the receiving plasmid.Notes
No internal BsaI sites.Source
All parts taken from Registry.| Sequence Annotation | Location | Component / Role(s) |
| Junction A P5 (J119031) Junction B BC spacer Junction C CD spacer Junction D | 1,18 19,54 55,72 73,76 77,94 95,98 99,116 | non_covalent_binding_site feature/binding feature/promoter promoter feature/binding non_covalent_binding_site feature/misc sequence_feature feature/binding non_covalent_binding_site sequence_feature feature/misc feature/binding non_covalent_binding_site |