Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: jessica gronniger
Date created: 2013-07-02 11:00:00
Date modified: 2015-08-31 04:08:23
sfGFP cloned into J100112
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_J100113_sequence (Version 1) |
Description
sfGFP (J100070) ligated between junctions C and D on scaffold (J119301). Golden Gate assembly was used to put the sfGFP into the gap between junctions C and D. iPCR was used to generate the receiving plasmid.Notes
No internal BsaI sites. The 5' end of Junction D from part BBa_J119301 was changed from TTAA to CGGA. The change was made by iPCR with primers that caused the amplicon to contain the new sequence as shown in part J100113.Source
All parts taken from Registry.| Sequence Annotation | Location | Component / Role(s) |
| Junction A P5 (J119031) Junction B C. dog BD24 (J119028) Junction C sfGFP (J100070) Junction D | 1,17 18,54 55,72 73,151 152,169 170,886 887,904 | non_covalent_binding_site feature/binding feature/protein CDS non_covalent_binding_site feature/binding ribosome_entry_site feature/rbs non_covalent_binding_site feature/binding sequence_feature feature/dna feature/binding non_covalent_binding_site |