Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: jessica gronniger
Date created: 2013-07-28 11:00:00
Date modified: 2015-08-31 04:08:23
J100116 with P5 Promoter Deleted
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_J100120_sequence (Version 1) |
Description
AdhE gene (J100109) was ligated between junctions C and D on scaffold (J119301). C. dog BD24 (J119028) was ligated between junctions B and C on scaffold. P5 promoter between junctions A and B was deleted. Golden Gate assembly was used to put the C. dog and adhE into the scaffold. iPCR was used to generate the receiving plasmid.Notes
No internal BsaI sites.Source
All parts taken from Registry.| Sequence Annotation | Location | Component / Role(s) |
| Junction A Junction B C. dog (J119028) Junction C adhE (J100109) Junction D | 1,17 18,35 36,115 116,133 134,2826 2827,2845 | feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding feature/rbs ribosome_entry_site feature/binding non_covalent_binding_site sequence_feature feature/dna non_covalent_binding_site feature/binding |