Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Phoebe Parrish, Meredith Simpson, A. Malcolm Campbell
Date created: 2013-07-30 11:00:00
Date modified: 2015-08-31 04:08:23
Promoter + Spinach + BsaI sites
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_J100121_sequence (Version 1) |
Description
This part contains a promoter, Spinach, and two BsaI sites with a spacer in between them. Spinach is a fluorogen-activated RNA sequence (biosensor) that binds to DFHBI and fluoresces green when a downstream mRNA sequence is produced. This part can be used with Golden Gate Assembly to determine whether a gene is being transcribed.Notes
This part was designed using iPCR to amplify the plasmid sent to us by CMU, removing FAP and the RBS. The iPCR primers contained BsaI and PstI sites with spacers in between. The iPCR products were digested using PstI, then ligated together.Source
The sequences were given to us by the Carnegie Mellon University 2012 iGEM team (http://2012.igem.org/Team:Carnegie_Mellon).| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter BsaI site Spinach BsaI site PstI site T7 terminator | 1,19 246,251 45,245 266,271 256,261 280,701 | feature/promoter promoter feature/binding non_covalent_binding_site feature/misc sequence_feature feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding feature/stop stop_codon |