Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2013-09-22 11:00:00
Date modified: 2015-08-31 04:08:23
human IL-6
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_J100131_sequence (Version 1) |
Description
Human IL-6 CDS optimized for E. coli with no internal restriction sites for EcoRI, XbaI, SpeI, PstI or BsaI. This insert was synthesized by GeneArt and was cloned into their pMK-T plasmid so we had the BioBrick prefix and suffix included in the basic part sequence. It will be used with Golden Gate Assembly to put downstream of promoter+RBS.Notes
Internal restriction sites removed for EcoRI, XbaI, SpeI, PstI or BsaI. This insert was synthesized by GeneArt and was cloned into their pMK-T plasmid so we had the BioBrick prefix and suffix included in the basic part sequence. It will be used with Golden Gate Assembly to put downstream of promoter+RBS.Source
came from GenBank and then was modified.| Sequence Annotation | Location | Component / Role(s) |
| Prefix Suffix start codon stop codon Human IL-6 BsaI cuts right sticky end BsaI cuts left sticky end | 1,22 603,623 34,36 589,591 34,591 23,28 30,33 597,602 592,595 | engineered_region feature/BioBrick feature/BioBrick engineered_region start_codon feature/start stop_codon feature/stop CDS feature/cds non_covalent_binding_site feature/binding feature/dna sequence_feature feature/binding non_covalent_binding_site sequence_feature feature/dna |