Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elizabeth Brunner
Date created: 2013-10-06 11:00:00
Date modified: 2015-08-31 04:08:23
thyA Gene
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_J100135_sequence (Version 1) |
Description
This construct includes promoter P5 + C-Dog BD24 (bi-cistronic RBS) + thyA gene (BBa_J100132). This gene is used to complement the mutation in E. coli strain C600thyA-::Kan^R (Thompson et al., 2002).Notes
There are no internal EcoRI, XbaI, SpeI, PstI, or BsaI restriction sites.Source
Promoter and C-Dog are from registry. ThyA is part BBa_J100132.| Sequence Annotation | Location | Component / Role(s) |
| P5 (strong) BD24 (strong C-Dog) C-Dog start thyA start thyA + 3 residues on N term. stop | 1,36 37,124 122,124 131,133 122,922 923,925 | promoter feature/promoter feature/rbs ribosome_entry_site feature/start start_codon start_codon feature/start CDS feature/cds feature/stop stop_codon |