Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2014-02-20 12:00:00
Date modified: 2015-08-31 04:08:23
T5+riboswitchD+thyA
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_J100136_sequence (Version 1) |
Description
This part has a constitutive promoter (T5) followed by theophylline riboswitch D followed by thyA gene. This is used as a fitness module in thyA- cells that can produce theophylline.Notes
We used golden gate assembly to put the thyA gene into T5+riboswitch into pSB1K8 plasmid. We used BsaI restriction enzyme.Source
composite device from existing parts| Sequence Annotation | Location | Component / Role(s) |
| T5 constitutive riboswitch D (theophylline) thyA gene stop start | 1,53 54,159 160,954 952,954 160,162 | feature/promoter promoter feature/rbs ribosome_entry_site CDS feature/cds stop_codon feature/stop start_codon feature/start |