BBa_J100166

BBa_J100166 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100166

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kathryn Smith

Date created: 2014-07-01 11:00:00

Date modified: 2015-08-31 04:08:23

P5 promoter + M6-R10 Ammeline Riboswitch + RFP

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_J100166_sequence (Version 1)

Description

This riboswitch sequence was built off of the original riboswitch sequence found in part J119335. It was designed to be a more effective riboswitch.
The sequence contains three mutations from the original part. Two of which were designed (C to G at 77 and a C insert at 183) and one which occurred accidentally (T to C at 564). The accidental mutation caused no amino acid change (E. coli codon bias change from .34 to .4).

Notes

n/a

Source

n/a

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P5 promoter	1,72	feature/promoter, promoter
M6''-R10 Ammeline Riboswitch	73,186	feature/misc, sequence_feature
RFP	187,871	feature/cds, CDS

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100166/1

Member of these Collections

• iGEM Parts Registry