BBa_J100172

BBa_J100172 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100172

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jonathan Lim

Date created: 2014-07-09 11:00:00

Date modified: 2015-08-31 04:08:23

Strong Promoter + M6"-R4 Ammeline Riboswitch + RFP

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_J100172_sequence (Version 1)

Description

This construct is a generic biosensor for ammeline, based on part J119335. The P5 promoter drives the M6"-R4 riboswitch, which permits translation of the reporter gene (RFP) in the presence of ammeline.

The M6"-R4 riboswitch is based on the M6" riboswitch, with a strong RBS (AGGAGAA) and sequence that is designed to completely sequester the Shine-Dalgarno sequence when ammeline is absent (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).

Mutation at position 430 nt of A to G changes tyrosine (Y) to cysteine (C) in RFP CDS.

Notes

N/A

Source

Part J119335 but replaced the M6" riboswitch with the newly designed M6"-R4 riboswitch.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
A432G from original RFP	432,432	sequence_alteration, feature/mutation
P5	19,54	promoter, feature/promoter
M6"-R4	73,190	sequence_feature, feature/misc
RFP	188,869	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100172/1

Member of these Collections

• iGEM Parts Registry