Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dustin Atchley
Date created: 2014-07-23 11:00:00
Date modified: 2015-08-31 04:08:23
Designed to optimize testing of fitness genes
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J100173_sequence (Version 1) |
Description
This construct is meant to be inserted into a plasmid backbone (stringent plasmid recommended). It is a theophylline riboswitch (D) and contains BsaI sites. After digesting with BsaI a fitness gene with the appropriately designed sticky ends can be inserted and tested.Notes
NASource
E. coli genome| Sequence Annotation | Location | Component / Role(s) |
| EcoRI J100065 (theophylline riboswitch D) T5 promoter Lac operator linkers and scars aptamer and actuator of riboswitch START BsaI <-- stuffer BsaI --> LVA Degradation Tag STOP PstI | 1,6 7,169 7,58 59,78 79,108 109,169 167,169 171,176 178,196 197,202 204,236 237,239 240,245 | non_covalent_binding_site feature/binding feature/misc sequence_feature promoter feature/promoter feature/operator operator feature/dna sequence_feature feature/binding non_covalent_binding_site feature/start start_codon sequence_feature feature/misc feature/dna sequence_feature sequence_feature feature/misc feature/tag tag feature/stop stop_codon feature/binding non_covalent_binding_site |