JEAN

BBa_J100183 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100183

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erin Xu, Nick Elder, Julia Preziosi, Abby Hunt

Date created: 2014-09-10 11:00:00

Date modified: 2015-08-31 04:08:23

P R/tetO

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_J100183_sequence (Version 1)

Description

This is a hybrid promoter that was engineered from a P R promote from rhizobial phage 16-3, along with its companion hybrid P R/tetO. It was engineered to be inducible by p-isopropyl benzoate (cumate)(Q) or anhydrotetracycline (aTc). P R/cmtO was observed to respond to a range of 0.1 to 5 ??g/ml (0.6 to 30 ??M) of Q and the P R/tetO promoter from 0.1 to 25 ng/ml (0.2 nM to 50 nM) aTc. This promoter was designed for use in Methylobacterium extorquens.

http://www.biomedcentral.com/1756-0500/6/183
"A novel pair of inducible expression vectors for use in Methylobacterium extorquens."

Notes

We designed the sticky ends to be inducible with BsaI.

Source

The genomic sequence comes from rhizobial phage 16-3.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10	42,47	non_covalent_binding_site, feature/binding
-35	22,27	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100183/1

Member of these Collections

• iGEM Parts Registry