BBa_J100184

BBa_J100184 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100184

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kevin Endersby

Date created: 2014-09-10 11:00:00

Date modified: 2015-08-31 04:08:23

Heat Induced Promoter on rpoD gene

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_J100184_sequence (Version 1)

Description

The Phs promoter on the rpoD gene allows the gene to still function under high heat. The rpoD gene codes for the sigma subunit of E. coli RNA polymerase. The Phs promoter functions ideally at 43 degrees Celsius.

Taylor, et. al. (1984) Cell v. 38 p. 371-381

Notes

The promoter functions ideally when placed for 3-10 minutes at 43 degrees Celsius. No Bsa I sites.

Source

E. coli

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35	17,22	non_covalent_binding_site, feature/binding
-10	40,45	non_covalent_binding_site, feature/binding

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100184/1

Member of these Collections

• iGEM Parts Registry