Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2014-10-23 11:00:00
Date modified: 2015-08-31 04:08:24
RBS (BD24) + GFP
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_J100201_sequence (Version 1) |
Description
This is a composite of superfolder GFP and a BD24 RBS (part BBa J119028). The stop codon of the RBS and start codon of GFP overlap to increase effective translation. There is also an EcoRI recognition site preceding the RBS.Notes
There are no BsaI sites.Source
We assembled the part from preexisting parts which were isolated and cloned together in one plasmid.| Sequence Annotation | Location | Component / Role(s) |
| EcoRI site RBS BD24 superfolding GFP A-->G T-->C Stop | 1,6 9,94 94,810 111,111 140,140 808,810 | feature/misc sequence_feature feature/rbs ribosome_entry_site feature/protein CDS sequence_alteration feature/mutation sequence_alteration feature/mutation feature/stop stop_codon |