Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2014-11-21 12:00:00
Date modified: 2015-08-31 04:08:24
Intermediate for IndClone Red
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J100202_sequence (Version 1) |
Description
We are building indClone Red to test inducers. This intermediate is a pSB1A8 plasmid containing RFP, RBS, and forward promoter. It will be an indicator of successful ligation of an inducer to the final plasmid.Notes
No BsaI sites so we will be able to later use Golden Gate Assembly.Source
E. coli-based genes| Sequence Annotation | Location | Component / Role(s) |
| partial Promoter R0082 RBS - BD21 (J119027) Red fluorescent protein & BD18 (strong RBS) E1010_BD18 PstI recognition site | 1,38 39,126 124,804 805,810 | feature/promoter promoter feature/rbs ribosome_entry_site CDS feature/protein non_covalent_binding_site feature/binding |