Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Camille Johnson
Date created: 2015-06-01 11:00:00
Date modified: 2015-08-31 04:08:24
tClone Red RS3
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J100207_sequence (Version 1) |
Description
We took out everything between the BsaI sites in J119361. We then inserted the RS3 riboswitch, the sequence of which was found in De novo design of a synthetic riboswitch that regulates transcription termination (Wachsmuth et. al).Notes
There is a BsaI site in the pUC IDT Amp plasmid that may confound results by cutting at a different site.Source
J119361; Wachsmuth et. al| Sequence Annotation | Location | Component / Role(s) |
| P5 Promoter RS3 Riboswitch BD18 C dog RBS RFP | 1,34 41,120 124,215 216,887 | feature/promoter promoter feature/stem_loop stem_loop ribosome_entry_site feature/rbs CDS feature/cds |