Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuk Hang Li
Date created: 2015-06-01 11:00:00
Date modified: 2015-08-31 04:08:24
tClone Red RS8
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J100209_sequence (Version 1) |
Description
This sequence has Isolated pUC-IDT from tClone Red (J119361) by removing sequence in between the BsaI restriction sites and then RS8 riboswtich (from Wachsmuth et al.) was ligated. A ligand such as theophylline can bind to the sequence and allow transcription to take place.Notes
BsaI recognition site in the pUC-IDT.Source
The receiving plasmid is J119361 and the riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013.| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter RS8 riboswtich BD18 C dog RBS RFP | 1,36 41,112 115,201 200,880 | feature/promoter promoter stem_loop feature/stem_loop ribosome_entry_site feature/rbs feature/cds CDS |