| Types | DnaRegion
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| Roles | Device
engineered_region
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Description
This part was made from adding the TetA gene from part J119140 in front of the RFP gene in the tCloneRed part J119361. The Tet and RFP genes were combined, by deleting the "stop" sequence in the Tet gene and adding a linker sequence of proteins GGGS x4 to create a fusion protein. The resultant protein is a single protein with both the Tetracycline resistance protein and the Red fluorescent protein, combined with a linker chain. This part still contains tClone, and will not transcribe the reporter gene (Tet+Red) without the insertion of a riboswitch etc. between the BsaI sites.
Notes
We did not know if the fusion protein would work with RFP as it was shown to do with GFP.
Source
The sources are J119140 and J119361. The linker chain was developed from part K598018 "tetA+GFP fused protein" by Qingyang XIAO.
