Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Preziosi
Date created: 2015-06-21 11:00:00
Date modified: 2015-08-31 04:08:24
tCloneTet+Red fusion protein reporter with Riboswitch 10Shift
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J100214_sequence (Version 1) |
Description
This is a hybrid taken from part J119386, with everything between the BsaI restriction sites removed, and a riboswitch put in its place. The riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013. This transcriptional riboswitch is expected to be able to regulate the reporter gene (in this case, the fusion protein Tet+RFP) which follows it. By binding to the ligand, theophylline, the riboswitch will be "on," and the gene will be expressed. The absence of theophylline should turn the switch "off" and the gene won't be expressed.Notes
The plasmid that this part is located in is pUC-BR, a plasmid modified from pUC-IDT-Amp with an errant BsaI site in the Ampicillin resistance gene removed.Source
Part BBa_J119386, and Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ . This is the same riboswitch as is included in part J100207.| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter Riboswitch 10 Shift TetA Linker sequence RFP BD18 C dog RBS | 1,36 41,127 217,1410 1411,1458 1459,2139 132,216 | feature/promoter promoter feature/stem_loop stem_loop CDS feature/cds CDS feature/cds feature/cds CDS feature/rbs ribosome_entry_site |