BBa_J100228

BBa_J100228 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100228

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sarah von Euler

Date created: 2015-09-09 11:00:00

Date modified: 2015-09-10 08:06:09

rpoD IPTG induced bacterial promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_J100228_sequence (Version 1)

Description

this promoter is found in phytoplasmas which are insect-transmissible and plant-pathogenic bacteria hat multiply intracellularly in plants and insects.
Chihiro Miura et.al.s via an in vitro transcription assay
This promoter is induced by 0.1 mM concentration of IPTG and binds with bacterial DNA. The -10 region is TATAAT and the -35 region is TTGCTA.

Notes

sticky ends were added to the strand which were CGAC on the left and CGCC on the right

Source

this promoter is found in phytoplasmas which are insect-transmissible and plant-pathogenic bacteria hat multiply intracellularly in plants and insects.
Chihiro Miura et.al.
Functional characterization of the principal sigma factor rpoD of phytoplasmas via an in vitro transcription assay

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10	44,49	promoter, feature/promoter
-35	21,26	promoter, feature/promoter

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_J100228/1

Member of these Collections

• iGEM Parts Registry