BBa_J100230

BBa_J100230 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100230

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shayna Moss

Date created: 2015-09-09 11:00:00

Date modified: 2015-09-10 08:07:14

HFQp:Cold-induced promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_J100230_sequence (Version 1)

Description

This promoter is cold-induced and it works between 4 and 20 degrees celsius. We will be putting this promoter sequence in a plasmid of E. coli to see if it expresses red fluorescent protein (RFP).

Notes

We had to add specific sticky ends for the BSA1 recognition sequence.

Source

It comes from an experiment done with multiple different promoters. HFQ protein.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10 box	37,42	feature/binding, non_covalent_binding_site
-35 box	9,14	feature/binding, non_covalent_binding_site

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100230/1

Member of these Collections

• iGEM Parts Registry