Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jeffrey Burton
Date created: 2016-01-28 12:00:00
Date modified: 2016-02-11 02:56:37
trp promotor lab
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J100250_sequence (Version 1) |
Description
We are inserting the TRP promoter into E. coli and monitoring the natural production of tryptophan. TRP is a repressible promoter which causes a negative feedback mechanism--the continued production of tryptophan with inhibit the TRP promoter which will then cut off the further production of tryptophan. We will observe the maximum threshold of the E. coli bacteria before the tryptophan levels increase enough to inhibit the TRP promoter.Notes
We had to take into consideration the natural production of tryptophan by the promoter which acts as a repressor when there are high concentrations of tryptophan.Source
http://www.ncbi.nlm.nih.gov/pubmed/11252809 where we found the TRP promoter and the -35 to -10 sequencehttp://www.hammiverse.com/lectures/18/4.html details of the negative loop mechanism