Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Helen Webster
Date created: 2016-03-23 12:00:00
Date modified: 2016-03-23 09:55:23
tCloneTet with RS10shift
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_J100252_sequence (Version 1) |
Description
Started with tCloneTet (J119367) and used Golden Gate Assembly to remove GFP reporter and put transcriptional terminator riboswitch RS10shift (RS10s). RS10s was taken from the Wachsmuth et. al. 2013 NAR vol. 41 p. 2541. This riboswitch works and is responsive to theophylline (2.5mM).Notes
Riboswitch cannot have internal Bsa1 restriction site.Source
Synthesized riboswitch and used J119367.| Sequence Annotation | Location | Component / Role(s) |
| P5 Promoter RS10shift Riboswitch BD18 C Dog RBS RFP protein half | 1,36 42,128 134,218 219,899 | promoter feature/promoter stem_loop feature/stem_loop ribosome_entry_site feature/rbs feature/protein CDS |