Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zachary Shaver
Date created: 2016-06-07 11:00:00
Date modified: 2016-06-08 10:13:19
rClone Red with Riboswitch E
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_J100262_sequence (Version 1) |
Description
This part is a modified form of the rClone Red plasmid (part BBa_J119384) that replaced the GFP section flanked by BsaI sites with a theophylline riboswitch, labeled riboswitch E, by Golden Gate Assembly (GGA). In the presence of theophylline, the cells should fluoresce red. In the absence of theophylline, the cells should not fluoresce.Notes
/Source
The sequence for riboswitch E was taken from the Gallivan et al. paper "A family of synthetic riboswitches adopts a kinetic trapping mechanism." The rClone Red plasmid used in GGA is part BBa_J119384.| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter Riboswitch E Red Fluorescent Protein | 1,36 41,94 101,781 | feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/cds |