Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Owen Koucky
Date created: 2016-06-08 11:00:00
Date modified: 2016-06-13 08:03:20
RS10 (Wachsmuth et al. 2013) in J119361
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_J100263_sequence (Version 1) |
Description
J11263 reports the functionality of the RS10 riboswitch from Wachsumth et al. 2013 by its expression of the reporter RFP gene. The RS10 riboswitch is a theophylline-binding transcriptional riboswitch that turns on gene expression in the presence of theophylline. In the presence of theophylline, cells containing this part should fluoresce red. In the absence of theophylline, cells containing this part should not fluoresce.Notes
The design of this sequence required the insertion of the RS10 riboswitch upstream of the reporter RFP gene. Such a design was made possible by the replacement of the GFP cassette in J119361 with the RS10 riboswitch via Golden Gate Assembly.Source
The RS10 riboswitch was developed by Wachsumth et al. for their 2013 paper "De novo design of a synthetic riboswitch that regulates transcription termination." The RS10 riboswitch was inserted into J119361 via Golden Gate Assembly and replaced the GFP cassette that had occupied this part.| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter RS10 BD18 C Dog RBS RFP protein half | 1,36 41,108 113,197 198,878 | promoter feature/promoter sequence_feature feature/misc ribosome_entry_site feature/rbs CDS feature/cds |