Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Owen Koucky
Date created: 2016-06-16 11:00:00
Date modified: 2016-06-17 07:34:16
pJC156c2 (Carlson et al. 2014)
| Types | DnaRegion |
| Roles | Intermediate engineered_region |
| Sequences | BBa_J100264_sequence (Version 1) |
Description
In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100264 when developing geneIII neg, the dominant negative allele of geneIII of the M13 phage.Notes
See "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014Source
"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014| Sequence Annotation | Location | Component / Role(s) |
| ColE1 Origin of Replication Rop PlacZ SD8 RBS geneIII (C83) Venus YFP aadA aadA promoter lacI lacIq | 1,589 1017,1208 1368,1524 1540,1554 1555,1872 1872,2591 2664,3445 3456,3540 3557,4639 4663,4706 | sequence_feature feature/misc CDS feature/cds feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/cds feature/misc sequence_feature CDS feature/cds promoter feature/promoter CDS feature/cds feature/promoter promoter |