BBa_J100264

BBa_J100264 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100264
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Owen Koucky
Date created: 2016-06-16 11:00:00
Date modified: 2016-06-17 07:34:16

pJC156c2 (Carlson et al. 2014)



Types
DnaRegion

Roles
Intermediate

engineered_region

Sequences BBa_J100264_sequence (Version 1)

Description

In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100264 when developing geneIII neg, the dominant negative allele of geneIII of the M13 phage.

Notes

See "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014

Source

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014

Sequence Annotation Location Component / Role(s)
ColE1 Origin of Replication
Rop
PlacZ
SD8 RBS
geneIII (C83)
Venus YFP
aadA
aadA promoter
lacI
lacIq
1,589
1017,1208
1368,1524
1540,1554
1555,1872
1872,2591
2664,3445
3456,3540
3557,4639
4663,4706
sequence_feature feature/misc
CDS feature/cds
feature/promoter promoter
ribosome_entry_site feature/rbs
CDS feature/cds
feature/misc sequence_feature
CDS feature/cds
promoter feature/promoter
CDS feature/cds
feature/promoter promoter
igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_J100264/1