Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Owen Koucky
Date created: 2016-06-16 11:00:00
Date modified: 2016-06-17 08:53:42
pJC173g-SD8_AP-neg (Carlson et al. 2014)
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_J100266_sequence (Version 1) |
Description
In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100266 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE).Notes
See "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014Source
"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014| Sequence Annotation | Location | Component / Role(s) |
| pUC Origin of Replication rrnB1 transcriptional terminator T7 promoter SD8 RBS geneIII neg Venus YFP aadA spectinomycin resistance aadA promoter | 1,799 815,1239 1264,1283 1305,1319 1320,2414 2414,3133 3213,4004 4005,4089 | sequence_feature feature/misc sequence_feature feature/misc promoter feature/promoter feature/rbs ribosome_entry_site feature/cds CDS feature/misc sequence_feature feature/cds CDS feature/promoter promoter |