BBa_J100268

BBa_J100268 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100268

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Owen Koucky

Date created: 2016-06-16 11:00:00

Date modified: 2016-06-17 02:07:15

pJC184d5

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_J100268_sequence (Version 1)

Description

In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100268 to be used as a drift plasmid in phage-assisted continuous evolution (PACE).

Notes

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014

Source

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cat (CmR) promoter	17,114	promoter, feature/promoter
cat (CmR) pJC184d5	115,774	sequence_feature, feature/misc
araC	881,1759	feature/cds, CDS
pBAD	1910,2062	feature/promoter, promoter
dnaQ926	2120,2851	CDS, feature/cds
umuD'	2889,3236	feature/cds, CDS
umuC	3239,4519	CDS, feature/cds
recA730	4583,5644	CDS, feature/cds
rrnB1 transcriptional terminator	5662,6086	sequence_feature, feature/misc
psp	6103,6232	sequence_feature, feature/misc
tetR binding site (175h)	6233,6251	feature/promoter, promoter
SD8 RBS	6307,6321	ribosome_entry_site, feature/rbs
geneIII	6322,7596	CDS, feature/cds
CloDF13	7753,8491	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100268/1

Member of these Collections

• iGEM Parts Registry