Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Buser
Date created: 2016-06-23 11:00:00
Date modified: 2016-06-24 10:14:45
tCloneTet+PDB riboswitch with P14 and BD10
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_J100277_sequence (Version 1) |
Description
The BD10 C Dog Ribosome Binding Site has a lower binding efficiency than BD18. PCR and Golden Gate Assembly was used to cut out the BD18 C Dog RBS of part J100274 and insert in the BD10 C Dog RBS.Notes
Since there was only a 6 base difference between BD18 C Dog and BD10 C Dog, the PCR was used to insert in and amplify the new BD10 C Dog RBS rather than during GGA. GGA was still necessary to make the dsDNA into a plasmid.Source
J100274| Sequence Annotation | Location | Component / Role(s) |
| P14 PDB riboswitch BD10 C Dog RBS TetA | 1,36 41,112 117,201 202,1398 | promoter feature/promoter feature/stem_loop stem_loop ribosome_entry_site feature/rbs CDS feature/cds |