Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zachary Shaver
Date created: 2016-07-05 11:00:00
Date modified: 2016-07-13 12:10:53
JC184d5 with Mutagenesis Cassette Removed
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_J100284_sequence (Version 1) |
Description
This part is the plasmid JC184d5 with the mutagenesis cassette removed. It is used in the negative selection phase of PACE to allow for survival of the desired phage with the addition of aTc. The mutagenesis cassette is removed to prevent a high rate of mutagenesis in the negative selection phase.Notes
All of the JC184d5 plasmid except the mutagenesis cassette was amplified using Gotaq Master Mix. The sequence was simply digested with the Not1 HF restriction enzyme and the two ends of the linearized plasmid were ligated together with NEB rapid ligase.Source
This part is a modified form of the JC184d5 plasmid that Davidson College received from Liu et al. (Harvard PACE group).| Sequence Annotation | Location | Component / Role(s) |
| cat CmR Promoter cat CmR recA730 rrnB1 Transcriptional Terminator psp tetR binding site (175h) SD8 RBS geneIII CloDF13 Origin of Replication rrnB1 Transcriptional Terminator | 17,114 115,774 874,875 893,1317 1334,1463 1464,1482 1538,1552 1553,2827 2984,3722 2850,2894 | promoter feature/promoter CDS feature/cds feature/cds CDS sequence_feature feature/misc sequence_feature feature/misc feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/cds feature/misc sequence_feature feature/misc sequence_feature |