BBa_J100299

BBa_J100299 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100299

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lydia Soifer

Date created: 2016-09-07 11:00:00

Date modified: 2016-09-08 08:11:24

lysine regulated promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_J100299_sequence (Version 1)

Description

This promoter includes the -91 to -81 regions directly upstream of the -35 to 0 region in addition to the sticky ends for BsaI.
Top1- 5' CGACTTTCACTGATATGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACG
Bot1- 5' CGCCCGTTTTCAGAGTAACCAGCGCATCGCTACGCCGCCATATCAGTGAAA
The -81 to -118 region is known to be a binding sight for lysine repression of the promoter (Bouvier, Jean, et. al 2008). If this whole region is not needed for lysine binding and the lysine will still bind if the binding sight is located directly upstream of the -35 region, the promoter should be activated in the absence of lysine and repressed in the presence of lysine.

Notes

See description.

Source

This sequence is from E. coli.
Bouvier, J., P. Strangler, V. Morales, E. Remy, and C. Gutierrez. "Lysine Represses Transcription of the E. Coli DapB Gene by Preventing Its Activation by the ArgP Activator." Journal of Bacteriology, Aug. 2008. p. 5224-5229.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10 box	35,40	feature/binding, non_covalent_binding_site
-35 box	12,17	non_covalent_binding_site, feature/binding
lysine repression site	1,11	feature/misc, sequence_feature

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100299/1

Member of these Collections

• iGEM Parts Registry